ASPOD modifications of 1993-1994

ASPOD, Autonomous Space Processors for Orbital Debris, provides a unique way of collecting the space debris that has built up over the past 37 years. For the past several years, ASPOD has gone through several different modifications. This year's concentrations were on the solar cutting array, the solar tracker, the earth based main frame/tilt table, the controls for the two robotic arms, and accurate autocad drawings of ASPOD. This final report contains the reports written by the students who worked on the ASPOD project this year

Parasitoid wasp controls blue gum psyllid

The blue gum psyllid was first discovered in North America in Monterey County in January 1991. Since then it has quickly spread throughout the California coastal area and has became a major pest on Eucalyptus palverulenta in commercial foliage plantations. Large amounts of pesticides have been used to control the psyllid in these plantations. A primary parasitoid wasp, Psyllaephagus pilosus Noyes (Hymenoptera: Encyrtidae), was found in Australia and New Zealand and released at eight sites in California in spring/summer 1993. As a result, psyllid populations declined somewhat at most sites in 1993, and in 1994 psyllids were no longer a problem. The parasitoid has spread rapidly to other coastal areas

Imported parasitic wasp helps control red gum lerp psyllid

The red gum lerp psyllid is an insect native to Australia, where it feeds upon eucalyptus species. Since 1998 this psyllid has spread throughout California, resulting in millions of dollars in damage and control costs. To help suppress the red gum lerp psyllid, a biological control program was initiated and a psyllid-specific parasitic wasp was imported from Australia in 1999 and released in 2000. In most coastal regions this biological control agent has provided substantial control, but in some interior regions the psyllid still remains a problem. Researchers are continuing their investigations to determine if full statewide suppression will be realized eventually, or if further importation of new parasitoid species is needed

A roadmap for high-throughput sequencing studies of wild animal populations using noninvasive samples and hybridization capture

Large-scale genomic studies of wild animal populations are often limited by access to high-quality DNA. Although noninvasive samples, such as faeces, can be readily collected, DNA from the sample producers is usually present in low quantities, fragmented, and contaminated by microorganism and dietary DNAs. Hybridization capture can help to overcome these impediments by increasing the proportion of subject DNA prior to high-throughput sequencing. Here we evaluate a key design variable for hybridization capture, the number of rounds of capture, by testing whether one or two rounds are most appropriate, given varying sample quality (as measured by the ratios of subject to total DNA). We used a set of 1,780 quality-assessed wild chimpanzee (Pan troglodytes schweinfurthii) faecal samples and chose 110 samples of varying quality for exome capture and sequencing. We used multiple regression to assess the effects of the ratio of subject to total DNA (sample quality), rounds of capture and sequencing effort on the number of unique exome reads sequenced. We not only show that one round of capture is preferable when the proportion of subject DNA in a sample is above ~2%–3%, but also explore various types of bias introduced by capture, and develop a model that predicts the sequencing effort necessary for a desired data yield from samples of a given quality. Thus, our results provide a useful guide and pave a methodological way forward for researchers wishing to plan similar hybridization capture studies.Funding was provided by the Max Planck Society and the President's Strategic Initiative Fund of ASU. T.M.B. is supported by BFU2017‐86471‐P (MINECO/FEDER, UE), U01 MH106874 grant, Howard Hughes International Early Career, Obra Social “La Caixa” and Secretaria d'Universitats i Recerca and CERCA Programme del Departament d'Economia i Coneixement de la Generalitat de Catalunya (GRC 2017 SGR 880) and C.F. is supported by a La Caixa PhD Fellowship